New progress has been made in the research
Recently, the team of Professor Zhichao Pei from the School of Chemistry and Pharmacy has made new progress in the field of cell chip research. The research results were published in "Sensors and Actuators B: Direct attachment of suspension cells to PDA surface and its application in suspension-cell QCM biosensor". Chemical" (2021, 326, 128823). The doctoral student Li Xueming of the school is the first author of the paper, and Professor Pei Zhichao is the corresponding author.
Quartz crystal microbalance (QCM)-based cell chip technology is an important tool for studying molecular-cell interactions and cell surface glycosylation. One of the primary problems of this technology is the need to stably fix cells on the chip surface. For adherent cells, the characteristics of their adherent growth can usually be used to make them adhere to the surface of the chip. However, for suspended cells, due to their suspended growth characteristics, it is more difficult to fix them directly on the surface of the chip. Polydopamine (PDA) has been widely used in the functionalization and modification of the surface of a variety of materials. At present, many articles have reported that PDA can promote the adhesion of adherent cells. However, it is still unclear whether suspension cells can adhere to the surface of PDA. There are reports. Inspired by mussel adhesion protein, Professor Pei Zhichao’s team conducted in-depth research on the adhesion performance of suspension cells on the surface of PDA, and for the first time successfully attached suspension cells directly to the surface of PDA, providing a way for the immobilization of suspension cells. new method. Using this method, the research team prepared a PDA-based suspension cell QCM chip. The chip preparation method is simple, efficient, and reusable. It can be used to detect the interaction between biomolecules and cells in real-time and label-free. Glycosylation on the cell surface and its complex molecular interactions provide a new method.
This research has won the National Natural Science Foundation of China (project number: 21877088, 21806067), the key construction project of the school discipline-sugar nanochemistry biomedicine cross-innovative research project (project number: 2017-C05) and the "double first-class" construction special fund, etc. Project funding.